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Get Price2.8. Liver tissue oxidative stress and cell factor assay. Liver tissue of 0.1 g was added to 1 mL cold saline and ground using high-throughput tissue grinder (Techin TJ-800D, China), centrifuged at 3000 rpm, and the supernatant was collected. Total protein concentration was measured using BCA Protein Assay Kit (Solarbio, China).
Get PriceIntroducing the Tissue Grinder Collection Kimble Chase provides much needed sample prep choices for researchers weary of the same old grind Finding the right combination of grinding surface and material is no longer a challenge for researchers who are preparing tissue samples for analysis. By putting together a broad line of tissue grinders, Kimble
Get Price6/29/2017 · Introducing a new and safer way to prepare differentials in the Hematology lab. Download Product Literature
Get Price3/29/2019 · Frozen tissues (25 mg) were homogenized for 30 s in PBS using tissue grinder (ACTGene, ACT-AG 3080). Homogenized tissues were cross-linked to final 1% formaldehyde, quenched with 125 mM glycine, and washed with TBS. The fixed homogenates were resuspended in cell lysis buffer and incubated on ice for 10 min.
Get Price6/17/2020 · After washing twice with 3.3× PBS 38 and dissociating the tissue in 2 ml 3.3× PBS using a 7-ml glass Dounce tissue grinder (Wheaton), another 3 ml 3.3× PBS was added.
Get PriceCommon Grinder Application. A New Gearbox Generation for Vertical Roller Mills. The first gearbox generation for vertical roller mills consisted of two helical gear stages combined with a bevel stage as shown in Fig. 3. This concept was used from 50 kW up to 3900 kW. Today, this concept is normally used up to a power of approx. 1000 kW, while ...
Get PriceTissue Grinders Teslon Pestle, PTFE Stainless Steel, ml ml; Individually Packed. For use in Preparation of Tissue Homogenates, etc. Autoclavable, Teon Pestle for Soft Tissue such as Brain or Liver, .~.mm Clearance between Mortar Pestle . No. Volume 91-00-001 Tissue Grinder E1, 1ml 91-00-020 E20, 20ml 91-00-030 E30, 30ml 91-00-050 E50, 50ml
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Get PriceThe NEW SpiralPestle™ and 1.5 ml Microtube grinder is an significant improvement over traditional smooth-walled polypropylene pestle and tube combinations. It is used to homogenize culture cells and small samples (<100 mg wet weight) of soft tissue such as liver, brain, and muscle. It can also be used to disperse centrifugal pellets, precipitates, and other solids.
Get PriceIn brief, 50 mg of leaf tissue was homogenized in a Kontes pellet pestle disposable tissue grinder (Thermo Fisher Scientific, Markhan, Ontario, Canada) in 200 μL of 80 : 20 (v/v) methanol (Fisher Optima LC/MS grade) : 0.1 N trichloroacetic acid (TCA).
Get Price5/17/2020 · The hospital where I work keeps several types of grinders on hand. Different types of tissue require different homogenization methods, so it is good to keep a variety of grinders in stock. One such type of grinder used to homogenize tissue is the kind with a hollow handle. This handle is meant to be packed full of ice.
Get PriceTissue Grinder, glass (1 ml) VWR or equivalent 14231-358 Tissue Grinder, glass (7 ml) VWR or equivalent 62400-620 ... pressing the plunger until the last bit of DNA has left the tip without introducing bubbles. Note: Removing the pipet tip from the gDNA solution too early, or improperly scraping the tip to break continuity ...
Get PriceComplete kit of six tissue grinders: 0.1, 0.2, 1, 1, 2, and 1 mL Packed in a lightweight protective carrying case Glass-to-glass ground surface homogenizes connective tissue. Teflon ® units suitable for soft tissue. Dounce Tissue Grinder is supplied with two interchangeable pestles for large and.
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Get Price6/28/2012 · The cells were then broken using a Pellet Pestle® tissue grinder (Kontes Glass Co., Vineland, NJ) with approximate 50 strokes on ice. One-ninth of the original cell resuspension volume of 10x Isolation Buffer (400 mM Tris-HCl, pH 7.4, 250 mM NaCl and 50 mM MgCl 2) was added to .
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Get Price7/30/2019 · The 293T SNORD32A knockout clone #8 was then used to create the double knockout by introducing the pair of SNORD51 sgRNAs. ... (Roche 4693116001) and homogenized with a glass dounce tissue grinder ...
Get PriceWe use a tissue grinder/homogenizer (from BDH) that has a glass mortar (tube) and a pestle with a hard plastic head. The clearance between pestle and mortar is 0.15-0.25 mm. Horizontal gel electrophoresis tank for agarose gels. Ice. Magnetic stirrer (see Step 32) Microcentrifuge tubes (1.5 ml and 2.0 ml)
Get PriceThere are three common routes by which leaf tissue is harvested prior to disruption. The first involves harvesting leaf tissue followed by freezing. Placing the tissue in a 80°C freezer provides a suitably cold environment that preserves DNA and many proteins, but is unsuitable for preserving RNA. Get + Grinder Machine Dna Extraction Plant floog
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